HCR-FISH visualization of collagen expression in P. waltl

Författare/Upphovsman:

Yodalr

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https://sewiki.info/b/c8341

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Top: Black and white bright-field microscopic view of four salamanders (Pleurodeles waltl) at developmental stages 35-37. Specimens 1 and 2 are captured ventrally, whereas specimens 3 and 4 are imaged dorsally. Among these, specimens 1, 3, and 4 are leucistic mutants, characterized by a knocked-out pigmentation gene, while specimen 2 is a wild-type, exhibiting no genetic mutations.

Bottom: The bottom panel illustrates the collagen expression patterns (CO2A1 gene) in these same salamanders, employing Hybridization Chain Reaction RNA Fluorescence In Situ Hybridization (HCR RNA-FISH) technology. The fluorescent visualization predominantly highlights the skeletal areas where collagen is abundantly present. Nonetheless, collagen expression is also observable in other regions, including the gills and on a distinct patch on top of the head.

The image was captured by Lennart Rikk from the Regenerative Immunology Lab (https://leighnd.github.io/) using Leica M205 FCA microscope.

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CC BY 4.0

Licensvillkor:

Creative Commons Attribution 4.0